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KMID : 0613820090190040457
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Journal of Life Science
2009 Volume.19 No. 4 p.457 ~ p.461
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Optimization of an Extracellular Dextranase Production from Lipomyces starkeyi KCTC 17343 and Analysis of Its Dextran Hydrolysates
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Chang Yoon-Hyuck
Jung Kyung-Hwan
Shin Jung-Hee
Yeom Joong-Hyun
Chang Byung-Chul
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Abstract
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We optimized dextranase culture conditions by batch fermentation using Lipomyces starkeyi KCTC 17343. Furthermore, dextranase was purified by an ultra-membrane, and then dextran hydrolyzates were characterized. Cell growth and dextranase production varied depending on the initial culture pH and temperature. The conditions of optimal dextranase production were met in a pH range of 4-5 and temperature between 25-30℃. At optimal fermentation conditions, total enzyme activity and specific enzyme activity were about 4.85 IU/㎖ and 0.79 IU/g cells, respectively. The specific growth rate was examined to be 0.076 hr-¹. The production of dextranase in culture broth was very stably maintained after mid-log phase of growth. The enzyme hydrolyzed dextran into DP (degree of polymerization) 2 to 8 oligodextran series. Analysis of the composition of hydrolysates suggested that the enzyme produced is an endo-dextranase.
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KEYWORD
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Lipomyces starkeyi, dextranase, dextran, fermentation, oligodextran
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